Coding

Part:BBa_K185047:Design

Designed by: Alex Jiang   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-16)

RelE toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a His-tag before the stop codon, then we mutated the stop codon from UAG(wild type) to UAA.

Source

Escherichia coli str. K-12 substr. DH10B, complete genome

References